FUJII L Nobuharu
教授
藤井 宣晴 フジイ ノブハル ふじい のぶはる
プロフィール
所属
東京都立大学人間健康科学研究科 ヘルスプロモーションサイエンス学域
大学教育センター
最終学歴・学位
筑波大学博士課程体育科学研究科 博士(体育科学)
専門・研究分野
分子生物学
研究
研究テーマ
運動が糖尿病の予防・改善に貢献するメカニズムの探索
筋収縮による細胞内情報伝達の変容解析
研究キーワード
骨格筋 筋収縮 糖尿病 AMPキナーゼ インスリン 運動 分子生物学 遺伝子工学
詳細情報
PDGF-B secreted from skeletal muscle enhances myoblast proliferation and myotube maturation via activation of the PDGFR signaling cascade. Hiroki Hamaguchi, Kitora Dohi, Takaomi Sakai, Masato Taoka, Toshiaki Isobe, Tsubasa S Matsui, Shinji Deguchi, Yasuro Furuichi, Nobuharu L Fujii, Yasuko Manabe. Biochemical and biophysical research communications 639 169-175 2023年1月8日 査読有り
Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate. Hiroki Hamaguchi, Tsubasa S. Matsui, Shinji Deguchi, Yasuro Furuichi, Nobuharu L. Fujii, Yasuko Manabe. Scientific Reports 12(1) 2022年8月15日 査読有り
R-spondin3 is a myokine that differentiates myoblasts to type I fibres. Yoshitaka Mita, Haonan Zhu, Yasuro Furuichi, Hiroki Hamaguchi, Yasuko Manabe, Nobuharu L Fujii. Scientific Reports 12(1) 13020-13020 2022年7月29日 査読有り
Mass spectrometry imaging reveals local metabolic changes in skeletal muscle due to chronic training. Naoko Goto-Inoue, Mizuki Morisasa, Keisuke Kimura, Tsukasa Mori, Yasuro Furuichi, Yasuko Manabe, Nobuharu L Fujii. Bioscience, Biotechnology, and Biochemistry 86(6) 730-738 2022年3月14日 査読有り
Effect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubes. Yoshitaka Mita, Miyuki Ito, Mio Yamada, Nobuharu L. Fujii, Yasuko Manabe, Yasuro Furuichi. The Journal of Physical Fitness and Sports Medicine 11(1) 51-56 2022年1月25日 査読有り
Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions. Yasuro Furuichi, Yuki Kawabata, Miho Aoki, Yoshitaka Mita, Nobuharu L. Fujii, Yasuko Manabe. Frontiers in Cell and Developmental Biology 9 2021年3月1日 査読有り
Effect of antioxidant supplementation on skeletal muscle and metabolic profile in aging mice. Sakuka Tsukamoto-Sen, Shinpei Kawakami, Hiroko Maruki-Uchida, Ryouichi Ito, Naoko Matsui, Yuki Komiya, Yoshitaka Mita, Mizuki Morisasa, Naoko Goto-Inoue, Yasuro Furuichi, Yasuko Manabe, Minoru Morita, Nobuharu L Fujii. Food & Function 12(2) 825-833 2021年1月21日 査読有り
Trans-omic Analysis Reveals ROS-Dependent Pentose Phosphate Pathway Activation after High-Frequency Electrical Stimulation in C2C12 Myotubes. Daisuke Hoshino, Kentaro Kawata, Katsuyuki Kunida, Atsushi Hatano, Katsuyuki Yugi, Takumi Wada, Masashi Fujii, Takanori Sano, Yuki Ito, Yasuro Furuichi, Yasuko Manabe, Yutaka Suzuki, Nobuharu L Fujii, Tomoyoshi Soga, Shinya Kuroda. iScience 23(10) 101558-101558 2020年10月23日 査読有り
Monitoring and mathematical modeling of mitochondrial ATP in myotubes at single-cell level reveals two distinct population with different kinetics
Naoki Matsuda, Ken-ichi Hironaka, Masashi Fujii, Takumi Wada, Katsuyuki Kunida, Haruki Inoue, Miki Eto, Daisuke Hoshino, Yasuro Furuichi, Yasuko Manabe, Nobuharu L. Fujii, Hiroyuki Noji, Hiromi Imamura, Shinya Kuroda. Quantitative Biology 8(3) 228-237 2020年9月 査読有り
Single-Cell Information Analysis Reveals That Skeletal Muscles Incorporate Cell-to-Cell Variability as Information Not Noise. Takumi Wada, Ken-Ichi Hironaka, Mitsutaka Wataya, Masashi Fujii, Miki Eto, Shinsuke Uda, Daisuke Hoshino, Katsuyuki Kunida, Haruki Inoue, Hiroyuki Kubota, Tsuguto Takizawa, Yasuaki Karasawa, Hirofumi Nakatomi, Nobuhito Saito, Hiroki Hamaguchi, Yasuro Furuichi, Yasuko Manabe, Nobuharu L Fujii, Shinya Kuroda. Cell Reports 32(9) 108051-108051 2020年9月1日 査読有り
Effect of treatment with conditioned media derived from C2C12 myotube on adipogenesis and lipolysis in 3T3-L1 adipocytes. Kotaro Tamura, Naoko Goto-Inoue, Kaede Miyata, Yasuro Furuichi, Nobuharu L Fujii, Yasuko Manabe. PLOS ONE 15(8) e0237095 2020年 査読有り
R3hdml regulates satellite cell proliferation and differentiation. Kenichi Sakamoto, Yasuro Furuichi, Masashi Yamamoto, Megumi Takahashi, Yoshihiro Akimoto, Takahiro Ishikawa, Takahiko Shimizu, Masanori Fujimoto, Aki Takada-Watanabe, Aiko Hayashi, Yoshitaka Mita, Yasuko Manabe, Nobuharu L Fujii, Ryoichi Ishibashi, Yoshiro Maezawa, Christer Betsholtz, Koutaro Yokote, Minoru Takemoto. EMBO Reports 20(11) e47957 2019年11月5日 査読有り
A new in vitro muscle contraction model and its application for analysis of mTORC1 signaling in combination with contraction and beta-hydroxy-beta-methylbutyrate administration. Satoko Sato, Mitsuru Nomura, Ikko Yamana, Akira Uchiyama, Yasuro Furuichi, Yasuko Manabe, Nobuharu L Fujii. Bioscience, Biotechnology, and Biochemistry 83(10) 1851-1857 2019年10月 査読有り
Characterization of myofiber-type-specific molecules using mass spectrometry imaging. Naoko Goto-Inoue, Mizuki Morisasa, Kazumasa Machida, Yasuro Furuichi, Nobuharu L Fujii, Shinji Miura, Tsukasa Mori. Rapid Communications in Mass Spectrometry : RCM 33(2) 185-192 2019年1月30日 査読有り
Reduced Dnmt3a increases Gdf5 expression with suppressed satellite cell differentiation and impaired skeletal muscle regeneration. Yukino Hatazawa, Yusuke Ono, Yuma Hirose, Sayaka Kanai, Nobuharu L Fujii, Shuichi Machida, Ichizo Nishino, Takahiko Shimizu, Masaki Okano, Yasutomi Kamei, Yoshihiro Ogawa. FASEB Journal : official publication of the Federation of American Societies for Experimental Biology 32(3) 1452-1467 2018年3月 査読有り
Mandai S, Furukawa S, Kodaka M, Hata Y, Mori T, Nomura N, Ando F, Mori Y, Takahashi D, Yoshizaki Y, Kasagi Y, Arai Y, Sasaki E, Yoshida S, Furuichi Y, Fujii NL, Sohara E, Rai T, Uchida S. Loop diuretics affect skeletal myoblast differentiation and exercise-induced muscle hypertrophy. Sci Rep. 18;7:46369. 2017
Kitamura K,Takamura Y,Iwamoto T,Nomura M,Iwasaki H,Ohdera M,Murakoshi M,Sugiyama K,Matsuyama K,Manabe Y,Fujii NL,Fushiki T: Dammarane-type triterpene extracts of Panax notoginseng root ameliorates hyperglycemia and insulin sensitivity by enhancing glucose uptake in skeletal muscle, Biosci Biotechnol Biochem,81(2):335-342, 2017
Inada A, Fujii NL, Inada O, Higaki Y, Furuichi Y, Nabeshima YI. Effects of 17β-Estradiol and Androgen on Glucose Metabolism in Skeletal Muscle. Endocrinology. 157: 4691-4705. 2016.
Inada A, Inada O, Fujii NL, Nagafuchi S, Katsuta H, Yasunami Y, Matsubara T, Arai H, Fukatsu A, Nabeshima YI. Adjusting the 17β-Estradiol-to-Androgen Ratio Ameliorates Diabetic Nephropathy. J Am Soc Nephrol. 27: 3035-3050,2016.
Goto-Inoue N, Tamura K, Motai F, Ito M, Miyata K, Manabe Y, Fujii NL: A fragmented form of annexin A1 is secreted from C2C12 myotubes by electric pulse-induced contraction, Mol Cell Biochem, 411, 173-180, 2016.
Manabe Y, Ogino S, Ito M, Furuichi Y, Takagi M, Yamada M, Goto-Inoue N, Ono Y, Fujii NL: Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes. Anal Biochem, 497: 36-38, 2016.
Yu H, Fujii NL, Toyoda T, An D, Farese RV, Leitges M, Hirshman MF, Mul JD, Goodyear LJ. Contraction stimulates muscle glucose uptake independent of atypical PKC. Physiol Rep 3(11) e12565 2015.
Manabe Y, Ogino S, Ito M, Furuichi Y, Takagi M, Yamada M, Goto-Inoue N, Ono Y, Fujii NL. Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes. Anal Biochem S0003-2697(15)00499-6. 2015.
Goto-Inoue N, Tamura K, Motai F, Ito M, Miyata K, Manabe Y, Fujii NL. A fragmented form of annexin A1 is secreted from C2C12 myotubes by electric pulse-induced contraction. Mol Cell Biochem in press 2015.
Shoji E, Sakurai H, Nishino T, Nakahata T, Heike T, Awaya T, Fujii N, Manabe Y, Matsuo M, Sehara-Fujisawa A. Early pathogenesis of Duchenne muscular dystrophy modelled in patient-derived human induced pluripotent stem cells. Sci Rep 5:12831. 2015
Manabe Y, Takagi M, Nakamura-Yamada M, Goto-Inoue N, Taoka M, Isobe T, Fujii NL. Redox proteins are constitutively secreted by skeletal muscle. J Physiol Sci 64(6):401-9. 2015
Inada A, Inada O, Fujii NL, Fujishima K, Inai T, Fujii H, Sueishi K, Kurachi K: β-cell induction in vivo in severely diabetic male mice by changing the circulating levels and pattern of the ratios of estradiol to androgens. Endocrinology. 155(10): 3829-42. 2014
Miyatake S, Manabe Y, Inagaki A, Furuichi Y, Takagi M, Taoka M, Isobe T, Hirota K, Fujii NL: Macrophage migration inhibitory factor diminishes muscle glucose transport induced by insulin and AICAR in a muscle type-dependent manner. Biochem Biophys Res Commun 444: 496-501, 2014.
Furuichi Y, Goto-Inoue N, Manabe Y, Setou M, Masuda K, Fujii NL: Imaging mass spectrometry reveals fiber-specific distribution of acetylcarnitine and contraction-induced carnitine dynamics in rat skeletal muscles. Biochim Biophys Acta 1837: 1699-1706, 2014.
Furuichi Y, Goto-Inoue N, Fujii NL: Role of carnitine acetylation in skeletal muscle. J Phys Fitness Sports Med 3: 163-168, 2014.
Manabe Y, Takagi M, Nakamura-Yamada M, Goto-Inoue N, Taoka M, Isobe T, Fujii NL: Redox proteins are constitutively secreted by skeletal muscle. J Physiol Sci 64: 401-409, 2014.
Inada A, Inada O, Fujii NL, Fujishima K, Inai T, Fujii H, Sueishi K, Kurachi K: β-cell induction in vivo in severely diabetic male mice by changing the circulating levels and pattern of the ratios of estradiol to androgens. Endocrinology 155: 3829-3842, 2014.
Furuichi Y, Goto-Inoue N, Fujii NL: Role of carnitine acetylation in skeletal muscle. J Phys Fitness Sports Med 3: 163-168, 2014.
Goto-Inoue N, Yamada K, Inagaki A, Furuichi Y, Ogino S, Manabe Y, Setou M, Fujii NL. Lipidomics analysis revealed the phospholipid compositional changes in muscle by chronic exercise and high-fat diet. Sci.Rep., 3, 3267, 2013
Manabe Y, Gollisch KSC, Holton L, Kim Y, Brandauer J, Fujii NL, Hirshman MF, Goodyear LJ. Exercise training-induced adaptations associated with increases in skeletal muscle glycogen content. FEBS J., 280(3):916-926, 2013
Tsuchiya M*, Manabe Y* (*contributed equally), Yamada K, Furuichi Y, Hosaka M, Fujii NL. Chronic exercise enhances insulin secretion ability of pancreatic islets without change in insulin content in non-diabetic rats. Biochem.Biophys.Res.Commun., 430(2):676-682, 2013
Manabe Y, Miyatake S, Takagi M, Nakamura M, Okeda A, Nakano T, Hirshman MF, Goodyear LJ, Fujii NL. Characterization of an Acute Muscle Contraction Model using Cultured C2C12 Myotubes. PLoS ONE,7(12):e52592, 2012
Goto-Inoue N, Setou M, Fujii NL. Visualization of metabolite change in skeletal muscle by contraction using imaging mass spectrometry. J.Phys.Fit.Sport.Med., 1(2): 347-350. 2012
Goto-Inoue N, Manabe Y, Miyatake S, Ogino S, Morishita A, Hayasaka T, Masaki N, Setou M, Fujii NL. Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry. Anal.Bioanal.Chem., 403(7):1863-1867, 2012
Toyoda T, An D, Witczak CA, Koh HJ, Hirshman MF, Fujii N, Goodyear LJ. Myo1c regulates glucose uptake in mouse skeletal muscle. J.Biol.Chem. 2011, 286(6):4133-40.
Koh HJ, Toyoda T, Fujii N, Jung MM, Rathod A, Middelbeek RJ, Lessard SJ, Treebak JT, Tsuchihara K, Esumi H, Richter EA, Wojtaszewski JF, Hirshman MF, and Goodyear LJ, Sucrose nonfermenting AMPK-related kinase (SNARK) mediates contraction-stimulated glucose transport in mouse skeletal muscle. Proc Natl.Acad.Sci. USA. 2010; 107(35): 15541-6.
Fujii N. Molecular mechanism of glucose transport in skeletal muscle. Adv.Exer.Sport.Physiol. 2008; 14(3): 45-47
Fujii N, Ho RC, Manabe Y, Jessen N, Toyoda T, Holland WL, Summers SA, Hirshman MF, Goodyear LJ. Ablation of AMP-activated protein kinase alpha2 activity exacerbates insulin resistance induced by high-fat feeding of mice. Diabetes. 2008 Nov;57(11):2958-66
Higaki Y, Mikami T, Fujii N, Hirshman MF, Koyama K, Seino T, Tanaka K, Goodyear LJ. Oxidative stress stimulates skeletal muscle glucose uptake through a phosphatidylinositol-3-kinase-dependent pathway. Am.J.Physiol. (Endocrinol Metab.) 2008; May;294(5):E889-97.
Taylor EB, An D, Kramer HF, Yu H, Fujii N, Roeckl KS, Bowles N, Hirshman MF, Xie J, Feener EP, Goodyear LJ. Discovery of TBC1D1 as an insulin-, AICAR-, and contraction-stimulated signaling nexus in mouse skeletal muscle. J.Biol.Chem. 2008; 283(15):9787-96.
Kramer HF, Taylor EB, Witczak CA, Fujii N, Hirshman MF, Goodyear LJ, Calmodulin-binding domain of AS160 regulates contraction- but not insulin-stimulated glucose uptake in skeletal muscle. Diabetes 2007; 56(12):2854-62.
Ho RC, *Fujii N, Witters LA, Hirshman MF, Goodyear LJ. Dissociation of AMP-activated protein kinase and p38 mitogen-activated protein kinase signaling in skeletal muscle.
Rockl KS, Hirshman MF, Brandauer J, Fujii N, Witters LA, Goodyear LJ. Skeletal muscle adaptation to exercise training: AMP-activated protein kinase mediates muscle fiber type shift. Diabetes 2007; 56(8):2062-9.
Fujii N, Seifert MM, Kane EM, Peter LE, Ho RC, Winstead S, Hirshman MF, Goodyear LJ. Role of AMP-activated protein kinase in exercise capacity, whole body glucose homeostasis, and glucose transport in skeletal suscle. - Insight from analysis of a transgenic mouse model - Diabetes Res.Clin.Pract. 2007; 77:S92-8.
Witczak CA, Fujii N, Hirshman MF, Goodyear LJ. Ca2+/calmodulin-dependent protein kinase kinase-alpha regulates skeletal muscle glucose uptake independent of AMP-activated protein kinase and Akt activation. Diabetes 2007; 56(5):1403-9.
Fujii N, Jessen N, Goodyear LJ. AMP-activated Protein Kinase and the Regulation of Glucose Transport. Am.J.Physiol. (Endcrinol.Metab.) 2006; 291(5):E867-77
Witczak CA, Hirshman MF, Jessen N, Fujii N, Seifert MM, Brandauer J, Hotamisligil GS, Goodyear LJ. JNK1 deficiency does not enhance muscle glucose metabolism in lean mice. Biochem.Biophys.Res.Commun. 2006; 350(4):1063-8
Koh H, Arnolds DE, Fujii N, Hirshman MF, Goodyear LJ. Skeletal muscle-selective knockout of LKB1 increases insulin sensitivity, improves glucose homeostasis, and decreases TRB3. Mol.Cell Biol. 2006; 26(22):8217-27
Kramer HF, Witczak CA, Taylor EB, Fujii N, Hirshman MF, Goodyear LJ. AS160 regulates insulin- and contraction-stimulated glucose uptake in mouse skeletal muscle. J.Biol.Chem. 2006; 281(42):31478-85
Kramer HF, Witczak CA, Fujii N, Jessen N, Taylor EB, Arnolds DE, Sakamoto K, Hirshman MF, Goodyear LJ. Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle. Diabetes 2006; 55(7):2067-76
Fujii N, Hirshman MF, Kane EM, Ho RC, Peter LE, Seifert MM, Goodyear LJ. AMP-activated protein kinase alpha2 activity is not essential for contraction-and hyperosmolarity-induced glucose transport in skeletal muscle. J.Biol.Chem. 2005; 280 (47): 29033-29041.
Fujii N, Aschenbach WG, Musi N, Hirshman MF, Goodyear LJ. Regulation of glucose transport by the AMP-activated protein kinase. Proc.Nutr.Soc. 2004; 63: 205-210
Alcazar O, Ho R, Fujii N, Goodyear LJ. cDNA cloning and functional characterization of a novel splice variant of c-Cbl associated protein (CAP) from mouse skeletal muscle. Biochem.Biophys.Res.Comm. 2004; 317(1): 285-93
Fujii N, Boppart MD, Dufresne SD, Crowley PF, Jozsi AC, Sakamoto K, Kim S, Miyazaki H, Hirshman MF, Goodyear LJ. Overexpression or ablatiation of JNK in skeletal muscle has no effect on glycogen synthase activity. Am.J.Physiol. (Cell Physiol.) 2004; 287(1): C200-8
Ho R, Alcazar O, Fujii N, Hirshman MF, Goodyear LJ. p38gamma MAPK regulation of glucose transporter expression and glucose uptake in L6 myotubes and mouse skeletal muscle. Am.J.Physiol. (Regul.Integr.Comp.Physiol.) 2004; 286(2): R342-9
Xing Y, Musi N, Fujii N, Zou L, Luptak I, Hirshman MF, Goodyear LJ, Tian R. Glucose metabolism and energy homeostasis in mouse hearts overexpressing dominant negative alpha 2 subunit of AMP-activated protein kinase. J.Biol.Chem.2003; 278(31): 28372-28377
Kaneto H, Xu G, Fujii N, Kim S, Bonner-Weir S, Weir GC. Involvement of c-Jun N-terminal kinase in oxidative stress-mediated suppression of insulin gene expression. J.Biol.Chem. 2002; 277(33): 30010-30018
Zhou G, Myers R, Li Y, Chen Y, Shen X, Fenyk-Melody J, Wu M, Ventre J, Doebber T, Fujii N, Musi N, Hirshman MF, Goodyear LJ, Moller DE. Role of AMP-activated protein kinase in mechanism of metformin action. J.Clin.Invest. 2001; 108(8): 1167-1174
Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate. Hiroki Hamaguchi, Tsubasa S. Matsui, Shinji Deguchi, Yasuro Furuichi, Nobuharu L. Fujii, Yasuko Manabe. Scientific Reports 12(1) 2022年8月15日 査読有り
R-spondin3 is a myokine that differentiates myoblasts to type I fibres. Yoshitaka Mita, Haonan Zhu, Yasuro Furuichi, Hiroki Hamaguchi, Yasuko Manabe, Nobuharu L Fujii. Scientific Reports 12(1) 13020-13020 2022年7月29日 査読有り
Mass spectrometry imaging reveals local metabolic changes in skeletal muscle due to chronic training. Naoko Goto-Inoue, Mizuki Morisasa, Keisuke Kimura, Tsukasa Mori, Yasuro Furuichi, Yasuko Manabe, Nobuharu L Fujii. Bioscience, Biotechnology, and Biochemistry 86(6) 730-738 2022年3月14日 査読有り
Effect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubes. Yoshitaka Mita, Miyuki Ito, Mio Yamada, Nobuharu L. Fujii, Yasuko Manabe, Yasuro Furuichi. The Journal of Physical Fitness and Sports Medicine 11(1) 51-56 2022年1月25日 査読有り
Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions. Yasuro Furuichi, Yuki Kawabata, Miho Aoki, Yoshitaka Mita, Nobuharu L. Fujii, Yasuko Manabe. Frontiers in Cell and Developmental Biology 9 2021年3月1日 査読有り
Effect of antioxidant supplementation on skeletal muscle and metabolic profile in aging mice. Sakuka Tsukamoto-Sen, Shinpei Kawakami, Hiroko Maruki-Uchida, Ryouichi Ito, Naoko Matsui, Yuki Komiya, Yoshitaka Mita, Mizuki Morisasa, Naoko Goto-Inoue, Yasuro Furuichi, Yasuko Manabe, Minoru Morita, Nobuharu L Fujii. Food & Function 12(2) 825-833 2021年1月21日 査読有り
Trans-omic Analysis Reveals ROS-Dependent Pentose Phosphate Pathway Activation after High-Frequency Electrical Stimulation in C2C12 Myotubes. Daisuke Hoshino, Kentaro Kawata, Katsuyuki Kunida, Atsushi Hatano, Katsuyuki Yugi, Takumi Wada, Masashi Fujii, Takanori Sano, Yuki Ito, Yasuro Furuichi, Yasuko Manabe, Yutaka Suzuki, Nobuharu L Fujii, Tomoyoshi Soga, Shinya Kuroda. iScience 23(10) 101558-101558 2020年10月23日 査読有り
Monitoring and mathematical modeling of mitochondrial ATP in myotubes at single-cell level reveals two distinct population with different kinetics
Naoki Matsuda, Ken-ichi Hironaka, Masashi Fujii, Takumi Wada, Katsuyuki Kunida, Haruki Inoue, Miki Eto, Daisuke Hoshino, Yasuro Furuichi, Yasuko Manabe, Nobuharu L. Fujii, Hiroyuki Noji, Hiromi Imamura, Shinya Kuroda. Quantitative Biology 8(3) 228-237 2020年9月 査読有り
Single-Cell Information Analysis Reveals That Skeletal Muscles Incorporate Cell-to-Cell Variability as Information Not Noise. Takumi Wada, Ken-Ichi Hironaka, Mitsutaka Wataya, Masashi Fujii, Miki Eto, Shinsuke Uda, Daisuke Hoshino, Katsuyuki Kunida, Haruki Inoue, Hiroyuki Kubota, Tsuguto Takizawa, Yasuaki Karasawa, Hirofumi Nakatomi, Nobuhito Saito, Hiroki Hamaguchi, Yasuro Furuichi, Yasuko Manabe, Nobuharu L Fujii, Shinya Kuroda. Cell Reports 32(9) 108051-108051 2020年9月1日 査読有り
Effect of treatment with conditioned media derived from C2C12 myotube on adipogenesis and lipolysis in 3T3-L1 adipocytes. Kotaro Tamura, Naoko Goto-Inoue, Kaede Miyata, Yasuro Furuichi, Nobuharu L Fujii, Yasuko Manabe. PLOS ONE 15(8) e0237095 2020年 査読有り
R3hdml regulates satellite cell proliferation and differentiation. Kenichi Sakamoto, Yasuro Furuichi, Masashi Yamamoto, Megumi Takahashi, Yoshihiro Akimoto, Takahiro Ishikawa, Takahiko Shimizu, Masanori Fujimoto, Aki Takada-Watanabe, Aiko Hayashi, Yoshitaka Mita, Yasuko Manabe, Nobuharu L Fujii, Ryoichi Ishibashi, Yoshiro Maezawa, Christer Betsholtz, Koutaro Yokote, Minoru Takemoto. EMBO Reports 20(11) e47957 2019年11月5日 査読有り
A new in vitro muscle contraction model and its application for analysis of mTORC1 signaling in combination with contraction and beta-hydroxy-beta-methylbutyrate administration. Satoko Sato, Mitsuru Nomura, Ikko Yamana, Akira Uchiyama, Yasuro Furuichi, Yasuko Manabe, Nobuharu L Fujii. Bioscience, Biotechnology, and Biochemistry 83(10) 1851-1857 2019年10月 査読有り
Characterization of myofiber-type-specific molecules using mass spectrometry imaging. Naoko Goto-Inoue, Mizuki Morisasa, Kazumasa Machida, Yasuro Furuichi, Nobuharu L Fujii, Shinji Miura, Tsukasa Mori. Rapid Communications in Mass Spectrometry : RCM 33(2) 185-192 2019年1月30日 査読有り
Reduced Dnmt3a increases Gdf5 expression with suppressed satellite cell differentiation and impaired skeletal muscle regeneration. Yukino Hatazawa, Yusuke Ono, Yuma Hirose, Sayaka Kanai, Nobuharu L Fujii, Shuichi Machida, Ichizo Nishino, Takahiko Shimizu, Masaki Okano, Yasutomi Kamei, Yoshihiro Ogawa. FASEB Journal : official publication of the Federation of American Societies for Experimental Biology 32(3) 1452-1467 2018年3月 査読有り
Mandai S, Furukawa S, Kodaka M, Hata Y, Mori T, Nomura N, Ando F, Mori Y, Takahashi D, Yoshizaki Y, Kasagi Y, Arai Y, Sasaki E, Yoshida S, Furuichi Y, Fujii NL, Sohara E, Rai T, Uchida S. Loop diuretics affect skeletal myoblast differentiation and exercise-induced muscle hypertrophy. Sci Rep. 18;7:46369. 2017
Kitamura K,Takamura Y,Iwamoto T,Nomura M,Iwasaki H,Ohdera M,Murakoshi M,Sugiyama K,Matsuyama K,Manabe Y,Fujii NL,Fushiki T: Dammarane-type triterpene extracts of Panax notoginseng root ameliorates hyperglycemia and insulin sensitivity by enhancing glucose uptake in skeletal muscle, Biosci Biotechnol Biochem,81(2):335-342, 2017
Inada A, Fujii NL, Inada O, Higaki Y, Furuichi Y, Nabeshima YI. Effects of 17β-Estradiol and Androgen on Glucose Metabolism in Skeletal Muscle. Endocrinology. 157: 4691-4705. 2016.
Inada A, Inada O, Fujii NL, Nagafuchi S, Katsuta H, Yasunami Y, Matsubara T, Arai H, Fukatsu A, Nabeshima YI. Adjusting the 17β-Estradiol-to-Androgen Ratio Ameliorates Diabetic Nephropathy. J Am Soc Nephrol. 27: 3035-3050,2016.
Goto-Inoue N, Tamura K, Motai F, Ito M, Miyata K, Manabe Y, Fujii NL: A fragmented form of annexin A1 is secreted from C2C12 myotubes by electric pulse-induced contraction, Mol Cell Biochem, 411, 173-180, 2016.
Manabe Y, Ogino S, Ito M, Furuichi Y, Takagi M, Yamada M, Goto-Inoue N, Ono Y, Fujii NL: Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes. Anal Biochem, 497: 36-38, 2016.
Yu H, Fujii NL, Toyoda T, An D, Farese RV, Leitges M, Hirshman MF, Mul JD, Goodyear LJ. Contraction stimulates muscle glucose uptake independent of atypical PKC. Physiol Rep 3(11) e12565 2015.
Manabe Y, Ogino S, Ito M, Furuichi Y, Takagi M, Yamada M, Goto-Inoue N, Ono Y, Fujii NL. Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes. Anal Biochem S0003-2697(15)00499-6. 2015.
Goto-Inoue N, Tamura K, Motai F, Ito M, Miyata K, Manabe Y, Fujii NL. A fragmented form of annexin A1 is secreted from C2C12 myotubes by electric pulse-induced contraction. Mol Cell Biochem in press 2015.
Shoji E, Sakurai H, Nishino T, Nakahata T, Heike T, Awaya T, Fujii N, Manabe Y, Matsuo M, Sehara-Fujisawa A. Early pathogenesis of Duchenne muscular dystrophy modelled in patient-derived human induced pluripotent stem cells. Sci Rep 5:12831. 2015
Manabe Y, Takagi M, Nakamura-Yamada M, Goto-Inoue N, Taoka M, Isobe T, Fujii NL. Redox proteins are constitutively secreted by skeletal muscle. J Physiol Sci 64(6):401-9. 2015
Inada A, Inada O, Fujii NL, Fujishima K, Inai T, Fujii H, Sueishi K, Kurachi K: β-cell induction in vivo in severely diabetic male mice by changing the circulating levels and pattern of the ratios of estradiol to androgens. Endocrinology. 155(10): 3829-42. 2014
Miyatake S, Manabe Y, Inagaki A, Furuichi Y, Takagi M, Taoka M, Isobe T, Hirota K, Fujii NL: Macrophage migration inhibitory factor diminishes muscle glucose transport induced by insulin and AICAR in a muscle type-dependent manner. Biochem Biophys Res Commun 444: 496-501, 2014.
Furuichi Y, Goto-Inoue N, Manabe Y, Setou M, Masuda K, Fujii NL: Imaging mass spectrometry reveals fiber-specific distribution of acetylcarnitine and contraction-induced carnitine dynamics in rat skeletal muscles. Biochim Biophys Acta 1837: 1699-1706, 2014.
Furuichi Y, Goto-Inoue N, Fujii NL: Role of carnitine acetylation in skeletal muscle. J Phys Fitness Sports Med 3: 163-168, 2014.
Manabe Y, Takagi M, Nakamura-Yamada M, Goto-Inoue N, Taoka M, Isobe T, Fujii NL: Redox proteins are constitutively secreted by skeletal muscle. J Physiol Sci 64: 401-409, 2014.
Inada A, Inada O, Fujii NL, Fujishima K, Inai T, Fujii H, Sueishi K, Kurachi K: β-cell induction in vivo in severely diabetic male mice by changing the circulating levels and pattern of the ratios of estradiol to androgens. Endocrinology 155: 3829-3842, 2014.
Furuichi Y, Goto-Inoue N, Fujii NL: Role of carnitine acetylation in skeletal muscle. J Phys Fitness Sports Med 3: 163-168, 2014.
Goto-Inoue N, Yamada K, Inagaki A, Furuichi Y, Ogino S, Manabe Y, Setou M, Fujii NL. Lipidomics analysis revealed the phospholipid compositional changes in muscle by chronic exercise and high-fat diet. Sci.Rep., 3, 3267, 2013
Manabe Y, Gollisch KSC, Holton L, Kim Y, Brandauer J, Fujii NL, Hirshman MF, Goodyear LJ. Exercise training-induced adaptations associated with increases in skeletal muscle glycogen content. FEBS J., 280(3):916-926, 2013
Tsuchiya M*, Manabe Y* (*contributed equally), Yamada K, Furuichi Y, Hosaka M, Fujii NL. Chronic exercise enhances insulin secretion ability of pancreatic islets without change in insulin content in non-diabetic rats. Biochem.Biophys.Res.Commun., 430(2):676-682, 2013
Manabe Y, Miyatake S, Takagi M, Nakamura M, Okeda A, Nakano T, Hirshman MF, Goodyear LJ, Fujii NL. Characterization of an Acute Muscle Contraction Model using Cultured C2C12 Myotubes. PLoS ONE,7(12):e52592, 2012
Goto-Inoue N, Setou M, Fujii NL. Visualization of metabolite change in skeletal muscle by contraction using imaging mass spectrometry. J.Phys.Fit.Sport.Med., 1(2): 347-350. 2012
Goto-Inoue N, Manabe Y, Miyatake S, Ogino S, Morishita A, Hayasaka T, Masaki N, Setou M, Fujii NL. Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry. Anal.Bioanal.Chem., 403(7):1863-1867, 2012
Toyoda T, An D, Witczak CA, Koh HJ, Hirshman MF, Fujii N, Goodyear LJ. Myo1c regulates glucose uptake in mouse skeletal muscle. J.Biol.Chem. 2011, 286(6):4133-40.
Koh HJ, Toyoda T, Fujii N, Jung MM, Rathod A, Middelbeek RJ, Lessard SJ, Treebak JT, Tsuchihara K, Esumi H, Richter EA, Wojtaszewski JF, Hirshman MF, and Goodyear LJ, Sucrose nonfermenting AMPK-related kinase (SNARK) mediates contraction-stimulated glucose transport in mouse skeletal muscle. Proc Natl.Acad.Sci. USA. 2010; 107(35): 15541-6.
Fujii N. Molecular mechanism of glucose transport in skeletal muscle. Adv.Exer.Sport.Physiol. 2008; 14(3): 45-47
Fujii N, Ho RC, Manabe Y, Jessen N, Toyoda T, Holland WL, Summers SA, Hirshman MF, Goodyear LJ. Ablation of AMP-activated protein kinase alpha2 activity exacerbates insulin resistance induced by high-fat feeding of mice. Diabetes. 2008 Nov;57(11):2958-66
Higaki Y, Mikami T, Fujii N, Hirshman MF, Koyama K, Seino T, Tanaka K, Goodyear LJ. Oxidative stress stimulates skeletal muscle glucose uptake through a phosphatidylinositol-3-kinase-dependent pathway. Am.J.Physiol. (Endocrinol Metab.) 2008; May;294(5):E889-97.
Taylor EB, An D, Kramer HF, Yu H, Fujii N, Roeckl KS, Bowles N, Hirshman MF, Xie J, Feener EP, Goodyear LJ. Discovery of TBC1D1 as an insulin-, AICAR-, and contraction-stimulated signaling nexus in mouse skeletal muscle. J.Biol.Chem. 2008; 283(15):9787-96.
Kramer HF, Taylor EB, Witczak CA, Fujii N, Hirshman MF, Goodyear LJ, Calmodulin-binding domain of AS160 regulates contraction- but not insulin-stimulated glucose uptake in skeletal muscle. Diabetes 2007; 56(12):2854-62.
Ho RC, *Fujii N, Witters LA, Hirshman MF, Goodyear LJ. Dissociation of AMP-activated protein kinase and p38 mitogen-activated protein kinase signaling in skeletal muscle.
Rockl KS, Hirshman MF, Brandauer J, Fujii N, Witters LA, Goodyear LJ. Skeletal muscle adaptation to exercise training: AMP-activated protein kinase mediates muscle fiber type shift. Diabetes 2007; 56(8):2062-9.
Fujii N, Seifert MM, Kane EM, Peter LE, Ho RC, Winstead S, Hirshman MF, Goodyear LJ. Role of AMP-activated protein kinase in exercise capacity, whole body glucose homeostasis, and glucose transport in skeletal suscle. - Insight from analysis of a transgenic mouse model - Diabetes Res.Clin.Pract. 2007; 77:S92-8.
Witczak CA, Fujii N, Hirshman MF, Goodyear LJ. Ca2+/calmodulin-dependent protein kinase kinase-alpha regulates skeletal muscle glucose uptake independent of AMP-activated protein kinase and Akt activation. Diabetes 2007; 56(5):1403-9.
Fujii N, Jessen N, Goodyear LJ. AMP-activated Protein Kinase and the Regulation of Glucose Transport. Am.J.Physiol. (Endcrinol.Metab.) 2006; 291(5):E867-77
Witczak CA, Hirshman MF, Jessen N, Fujii N, Seifert MM, Brandauer J, Hotamisligil GS, Goodyear LJ. JNK1 deficiency does not enhance muscle glucose metabolism in lean mice. Biochem.Biophys.Res.Commun. 2006; 350(4):1063-8
Koh H, Arnolds DE, Fujii N, Hirshman MF, Goodyear LJ. Skeletal muscle-selective knockout of LKB1 increases insulin sensitivity, improves glucose homeostasis, and decreases TRB3. Mol.Cell Biol. 2006; 26(22):8217-27
Kramer HF, Witczak CA, Taylor EB, Fujii N, Hirshman MF, Goodyear LJ. AS160 regulates insulin- and contraction-stimulated glucose uptake in mouse skeletal muscle. J.Biol.Chem. 2006; 281(42):31478-85
Kramer HF, Witczak CA, Fujii N, Jessen N, Taylor EB, Arnolds DE, Sakamoto K, Hirshman MF, Goodyear LJ. Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle. Diabetes 2006; 55(7):2067-76
Fujii N, Hirshman MF, Kane EM, Ho RC, Peter LE, Seifert MM, Goodyear LJ. AMP-activated protein kinase alpha2 activity is not essential for contraction-and hyperosmolarity-induced glucose transport in skeletal muscle. J.Biol.Chem. 2005; 280 (47): 29033-29041.
Fujii N, Aschenbach WG, Musi N, Hirshman MF, Goodyear LJ. Regulation of glucose transport by the AMP-activated protein kinase. Proc.Nutr.Soc. 2004; 63: 205-210
Alcazar O, Ho R, Fujii N, Goodyear LJ. cDNA cloning and functional characterization of a novel splice variant of c-Cbl associated protein (CAP) from mouse skeletal muscle. Biochem.Biophys.Res.Comm. 2004; 317(1): 285-93
Fujii N, Boppart MD, Dufresne SD, Crowley PF, Jozsi AC, Sakamoto K, Kim S, Miyazaki H, Hirshman MF, Goodyear LJ. Overexpression or ablatiation of JNK in skeletal muscle has no effect on glycogen synthase activity. Am.J.Physiol. (Cell Physiol.) 2004; 287(1): C200-8
Ho R, Alcazar O, Fujii N, Hirshman MF, Goodyear LJ. p38gamma MAPK regulation of glucose transporter expression and glucose uptake in L6 myotubes and mouse skeletal muscle. Am.J.Physiol. (Regul.Integr.Comp.Physiol.) 2004; 286(2): R342-9
Xing Y, Musi N, Fujii N, Zou L, Luptak I, Hirshman MF, Goodyear LJ, Tian R. Glucose metabolism and energy homeostasis in mouse hearts overexpressing dominant negative alpha 2 subunit of AMP-activated protein kinase. J.Biol.Chem.2003; 278(31): 28372-28377
Kaneto H, Xu G, Fujii N, Kim S, Bonner-Weir S, Weir GC. Involvement of c-Jun N-terminal kinase in oxidative stress-mediated suppression of insulin gene expression. J.Biol.Chem. 2002; 277(33): 30010-30018
Zhou G, Myers R, Li Y, Chen Y, Shen X, Fenyk-Melody J, Wu M, Ventre J, Doebber T, Fujii N, Musi N, Hirshman MF, Goodyear LJ, Moller DE. Role of AMP-activated protein kinase in mechanism of metformin action. J.Clin.Invest. 2001; 108(8): 1167-1174
2006年7月Travel Award Federation of American Societies for Experimental Biology (FASEB)
藤井 宣晴
2001年4月Iacocca Research Fellowship The Mary K. Iacocca Foundation
藤井 宣晴
1999年4月海外特別研究員 日本学術振興会
藤井 宣晴
1999年4月Fellowship for Research Abroad 辞退 上原記念財団
藤井 宣晴
1996年1月特別研究員 PD 日本学術振興会
藤井 宣晴
藤井 宣晴
2001年4月Iacocca Research Fellowship The Mary K. Iacocca Foundation
藤井 宣晴
1999年4月海外特別研究員 日本学術振興会
藤井 宣晴
1999年4月Fellowship for Research Abroad 辞退 上原記念財団
藤井 宣晴
1996年1月特別研究員 PD 日本学術振興会
藤井 宣晴
著書等
骨格筋と他臓器の連関 4.マイオカイン研究がめざす先. 藤井宣晴, 三田佳貴. 実験医学 40(2) 2022年
シン・骨格筋ワールド:< 藤井宣晴 実験医学 40(2) 2022年
骨格筋の「質」を制御するマイオカイン 古市泰郎, 三田佳貴, 眞鍋康子, 藤井宣晴 体力科学 71(1) 2022年
肥満症と疾患 4.脂肪過多と筋過少が同居した状態:サルコペニア肥満 藤井宣晴, 古市泰郎, 眞鍋康子 実験医学 39(5) 2021年
食事療法と運動療法 II.運動療法の基礎と臨床 2.運動とマイオカインと糖・脂質代謝 眞鍋康子, 藤井宣晴 Lipid 32(1) 2021年
糖尿病診療における運動・身体活動 5 マイオカインと骨格筋分子生物学 藤井宣晴, 眞鍋康子, 古市泰郎 月刊糖尿病 13(9) 2021年
筋幹細胞の増殖におけるグルコースの意義 古市泰郎, 川端有紀, 眞鍋康子, 藤井宣晴 日本筋学会学術集会プログラム・抄録集 7th 2021年
骨格筋の生物学-運動療法のサイエンスも踏まえて マイオカインと骨格筋分子生物学 藤井 宣晴 糖尿病 63(Suppl.1) S-78 2020年8月
骨格筋研究がつくるスポーツ科学の新たな価値 藤井 宣晴 Journal of Exercise Science 29 16-18 2020年3月
マイオカインを運動療法に利用するための方策 藤井宣晴, 古市泰郎, 眞鍋康子 糖尿病学の進歩 54th 2020年
マイオカインと骨格筋分子生物学 藤井宣晴 糖尿病(Web) 63(Suppl) 2020年
身体運動による健康効果にマイオカインが果たす役割 藤井 宣晴 上原記念生命科学財団研究報告集 33 1-4 2019年12月
健康寿命延伸に寄与する体力医学(第11回) 加齢に伴う骨・骨格筋量減少に及ぼす運動の影響 そのメカニズム 古市 泰郎, 藤井 宣晴 医学のあゆみ 269(12) 949-954 2019年6月
骨格筋と他臓器の連関 4.マイオカイン研究がめざす先. 藤井宣晴, 三田佳貴. 実験医学 40(2) 2022年
シン・骨格筋ワールド:< 藤井宣晴 実験医学 40(2) 2022年
骨格筋の「質」を制御するマイオカイン 古市泰郎, 三田佳貴, 眞鍋康子, 藤井宣晴 体力科学 71(1) 2022年
肥満症と疾患 4.脂肪過多と筋過少が同居した状態:サルコペニア肥満 藤井宣晴, 古市泰郎, 眞鍋康子 実験医学 39(5) 2021年
食事療法と運動療法 II.運動療法の基礎と臨床 2.運動とマイオカインと糖・脂質代謝 眞鍋康子, 藤井宣晴 Lipid 32(1) 2021年
糖尿病診療における運動・身体活動 5 マイオカインと骨格筋分子生物学 藤井宣晴, 眞鍋康子, 古市泰郎 月刊糖尿病 13(9) 2021年
筋幹細胞の増殖におけるグルコースの意義 古市泰郎, 川端有紀, 眞鍋康子, 藤井宣晴 日本筋学会学術集会プログラム・抄録集 7th 2021年
骨格筋の生物学-運動療法のサイエンスも踏まえて マイオカインと骨格筋分子生物学 藤井 宣晴 糖尿病 63(Suppl.1) S-78 2020年8月
骨格筋研究がつくるスポーツ科学の新たな価値 藤井 宣晴 Journal of Exercise Science 29 16-18 2020年3月
マイオカインを運動療法に利用するための方策 藤井宣晴, 古市泰郎, 眞鍋康子 糖尿病学の進歩 54th 2020年
マイオカインと骨格筋分子生物学 藤井宣晴 糖尿病(Web) 63(Suppl) 2020年
身体運動による健康効果にマイオカインが果たす役割 藤井 宣晴 上原記念生命科学財団研究報告集 33 1-4 2019年12月
健康寿命延伸に寄与する体力医学(第11回) 加齢に伴う骨・骨格筋量減少に及ぼす運動の影響 そのメカニズム 古市 泰郎, 藤井 宣晴 医学のあゆみ 269(12) 949-954 2019年6月
- 人間健康科学概論
- 環境生理生化学
- 代謝栄養学演習
- ヘルスプロモーションサイエンス特別研究a
- ヘルスプロモーションサイエンス研究法Ⅰ
- ヘルスプロモーションサイエンス研究法Ⅱ
- 適応科学特別講義
- 運動分子生物学特論
- 運動生理学特論
- ヘルスプロモーションサイエンス特別研究a
- ヘルスプロモーションサイエンス特講Ⅰ
- ヘルスプロモーションサイエンス特別講義Ⅰ
- ヘルスプロモーションサイエンス特別研究a
- ヘルスプロモーションサイエンス特別研究b
- ヘルスプロモーションサイエンス特別研究b
- ヘルスプロモーションサイエンス特別研究a
- ヘルスプロモーションサイエンス特別研究b
- ヘルスプロモーションサイエンス特別研究b
- 研究室インターンシップ(生体理工学)
- 研究室インターンシップ(生体理工学)
- 運動生命医科学
- 身体運動学
- 身体運動演習(I3)
- 組織再編前旧課程の同時開講科目等が含まれており、掲載されている全ての科目を開講するわけではありません。
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