本文へ移動します

廣田 耕志

氏 名廣田 耕志ヒロタ コウジひろた こうじ 
職 位教授
所 属東京都立大学理学部 化学科
理学研究科 化学専攻
2018年再編前の所属
都市教養学部 理工学系 化学コース
理工学研究科 分子物質化学専攻
 
専門・研究分野生物化学
最終学歴・学位東京大学大学院理学系研究科生物化学専攻・博士(理学)
研究テーマ染色体恒常性維持機構の解明
研究キーワード染色体 クロマチン DNA修復 ガン 環境遺伝毒物
研究業績・著書・
論文、その他
それに準じる業績
1. Senmatsu S, Asada R, Abe T, Hoffman CS, Ohta K, Hirota K lncRNA transcriptional initiation induces chromatin remodeling within a limited range in the fission yeast fbp1 promoter. Sci Rep 9: 299 (2019)
2. Tsuda M, Ogawa S, Ooka M, Kobayashi K, Hirota K, Wakasugi M, Matsunaga T, Sakuma T, Yamamoto T, Chikuma S, Sasanuma H, Debatisse M, Doherty AJ, Fuchs RP, Takeda S. PDIP38/PolDIP2 controls the DNA damage tolerance pathways by increasing the relative usage of translesion DNA synthesis over template switching. PLoS One. 6 e0213383. doi: 10.1371/journal.pone.0213383. eCollection (2019)
3. Abe T, Branzei D, Hirota K DNA Damage Tolerance Mechanisms Revealed from the Analysis of Immunoglobulin V Gene Diversification in Avian DT40 Cells. Genes (Basel) 9(2018)
4. Umeda M, Tsunekawa C, Senmatsu S, Asada R, Abe T, Ohta K, Hoffman CS, Hirota K Histone-chaperone Asf1 is required for the establishment of repressive chromatin in Schizosaccharomyces pombe fbp1 gene repression. Mol Cell Biol (2018)
5. Saha LK, Kim S, Kang H, Akter S, Choi K, Sakuma T, Yamamoto T, Sasanuma H, Hirota K, Nakamura J, Honma M, Takeda S, Dertinger S Differential micronucleus frequency in isogenic human cells deficient in DNA repair pathways is a valuable indicator for evaluating genotoxic agents and their genotoxic mechanisms. Environ Mol Mutagen (2018)
6. Ooka M, Abe T, Cho K, Koike K, Takeda S, Hirota K Chromatin remodeler ALC1 prevents replication-fork collapse by slowing fork progression. PLoS One 13: e0192421 (2018)
7. Nakazato A, Kajita K, Ooka M, Akagawa R, Abe T, Takeda S, Branzei D, Hirota K SPARTAN promotes genetic diversification of the immunoglobulin-variable gene locus in avian DT40 cells. DNA Repair (Amst) 68: 50-57 (2018)
8. Adachi A, Senmatsu S, Asada R, Abe T, Hoffman CS, Ohta K, Hirota K Interplay between chromatin modulators and histone acetylation regulates the formation of accessible chromatin in the upstream regulatory region of fission yeast fbp1. Genes Genet Syst 92: 267-276 (2018)
9. Abe T, Ooka M, Kawasumi R, Miyata K, Takata M, Hirota K, Branzei D Warsaw breakage syndrome DDX11 helicase acts jointly with RAD17 in the repair of bulky lesions and replication through abasic sites. Proc Natl Acad Sci U S A 115: 8412-8417 (2018)
10. Abe T, Kawasumi R, Giannattasio M, Dusi S, Yoshimoto Y, Miyata K, Umemura K, Hirota K, Branzei D AND-1 fork protection function prevents fork resection and is essential for proliferation. Nat Commun 9: 3091 (2018)
11. Tsuda M, Terada K, Ooka M, Kobayashi K, Sasanuma H, Fujisawa R, Tsurimoto T, Yamamoto J, Iwai S, Kadoda K, Akagawa R, Huang SN, Pommier Y, Sale JE, Takeda S, Hirota K The dominant role of proofreading exonuclease activity of replicative polymerase epsilon in cellular tolerance to cytarabine (Ara-C). Oncotarget 8: 33457-33474 (2017)
12. Tsuda M, Cho K, Ooka M, Shimizu N, Watanabe R, Yasui A, Nakazawa Y, Ogi T, Harada H, Agama K, Nakamura J, Asada R, Fujiike H, Sakuma T, Yamamoto T, Murai J, Hiraoka M, Koike K, Pommier Y, Takeda S, Hirota K ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair. PLoS One 12: e0188320 (2017)
13. Kawasumi R, Abe T, Arakawa H, Garre M, Hirota K, Branzei D ESCO1/2's roles in chromosome structure and interphase chromatin organization. Genes Dev 31: 2136-2150 (2017)
14. Fujisawa R, Ohashi E, Hirota K, Tsurimoto T Human CTF18-RFC clamp-loader complexed with non-synthesising DNA polymerase epsilon efficiently loads the PCNA sliding clamp. Nucleic Acids Res 45: 4550-4563 (2017)
15. Asada R, Umeda M, Adachi A, Senmatsu S, Abe T, Iwasaki H, Ohta K, Hoffman CS, Hirota K Recruitment and delivery of the fission yeast Rst2 transcription factor via a local genome structure counteracts repression by Tup1-family corepressors. Nucleic Acids Res 45: 9361-9371 (2017)
16. Hirota K, Tsuda M, Mohiuddin, Tsurimoto T, Cohen IS, Livneh Z, Kobayashi K, Narita T, Nishihara K, Murai J, Iwai S, Guilbaud G, Sale JE, Takeda S In vivo evidence for translesion synthesis by the replicative DNA polymerase delta. Nucleic Acids Res 44: 7242-50 (2016)
17. Kobayashi K, Guilliam TA, Tsuda M, Yamamoto J, Bailey LJ, Iwai S, Takeda S, Doherty AJ, Hirota K Repriming by PrimPol is critical for DNA replication restart downstream of lesions and chain-terminating nucleosides. Cell Cycle 15: 1997-2008 (2016)
18. Ooka M, Kobayashi K, Abe T, Akiyama K, Hada M, Takeda S, Hirota K Determination of genotoxic potential by comparison of structurally related azo dyes using DNA repair-deficient DT40 mutant panels. Chemosphere 164: 106-112 (2016)
19. Ooka M, Takazawa H, Takeda S, Hirota K Cytotoxic and genotoxic profiles of benzopyrene and N-nitrosodimethylamine demonstrated using DNA repair deficient DT40 cells with metabolic activation. Chemosphere 144: 1901-7 (2016)
20. Takemata N, Oda A, Yamada T, Galipon J, Miyoshi T, Suzuki Y, Sugano S, Hoffman CS, Hirota K, Ohta K Local potentiation of stress-responsive genes by upstream noncoding transcription. Nucleic Acids Res 44: 5174-89 (2016)
21. Yamamoto J, Takahata C, Kuraoka I, Hirota K, Iwai S Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3'-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease. Molecules 21(2016)
22. Asada R, Takemata N, Hoffman CS, Ohta K, Hirota K Antagonistic controls of chromatin and mRNA start site selection by Tup family corepressors and the CCAAT-binding factor. Mol Cell Biol 35: 847-55 (2015)
23. Hirota K, Yoshikiyo K, Guilbaud G, Tsurimoto T, Murai J, Tsuda M, Phillips LG, Narita T, Nishihara K, Kobayashi K, Yamada K, Nakamura J, Pommier Y, Lehmann A, Sale JE, Takeda S The POLD3 subunit of DNA polymerase delta can promote translesion synthesis independently of DNA polymerase zeta. Nucleic Acids Res 43: 1671-83 (2015)
24. Hoa NN, Akagawa R, Yamasaki T, Hirota K, Sasa K, Natsume T, Kobayashi J, Sakuma T, Yamamoto T, Komatsu K, Kanemaki MT, Pommier Y, Takeda S, Sasanuma H Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines. Genes Cells 20: 1059-76 (2015)
25. Kobayashi K, Fujii T, Asada R, Ooka M, Hirota K Development of a targeted flip-in system in avian DT40 cells. PLoS One 10: e0122006 (2015)
26. Kobayashi S, Kasaishi Y, Nakada S, Takagi T, Era S, Motegi A, Chiu RK, Takeda S, Hirota K Rad18 and Rnf8 facilitate homologous recombination by two distinct mechanisms, promoting Rad51 focus formation and suppressing the toxic effect of nonhomologous end joining. Oncogene 34: 4403-11 (2015)
27. Shimizu N, Ooka M, Takagi T, Takeda S, Hirota K Distinct DNA Damage Spectra Induced by Ionizing Radiation in Normoxic and Hypoxic Cells. Radiat Res 184: 442-8 (2015)
28. Tada K, Kobayashi M, Takiuchi Y, Iwai F, Sakamoto T, Nagata K, Shinohara M, Io K, Shirakawa K, Hishizawa M, Shindo K, Kadowaki N, Hirota K, Yamamoto J, Iwai S, Sasanuma H, Takeda S, Takaori-Kondo A Abacavir, an anti-HIV-1 drug, targets TDP1-deficient adult T cell leukemia. Sci Adv 1: e1400203 (2015)
受 賞日本遺伝学会GGS prize 2018
主な学会活動分子生物学会 正会員
日本癌学会 正会員
日本環境変異原学会(JEMS)
社会等との関わりDNA損傷応答、損傷修復機構の解明のために基礎科学的研究を推進します。研究を通じ得られた知見をもとに新しいガン治療法を社会に提案します。
個人のURL
担当科目
  • 生体物質化学I
  • 生体物質化学II
  • 化学セミナー
  • 化学コロキウムI
  • 化学実験(化学科用)
  • 生体物質化学I
  • 生体物質化学II
  • 化学セミナー
  • 化学コロキウムI
  • 化学実験(化学コース用)
  • 化学特論IV(現代生命科学)
  • 化学特別セミナーI
  • 化学特別セミナーII
  • 化学特別セミナーIII
  • 化学特別セミナーIV
  • 化学特別セミナーII
  • 化学特別セミナーI
  • 化学特別セミナーIV
  • 化学特別セミナーIII
  • 化学特論IV(現代生命科学)
  • 化学特別セミナーI
  • 化学特別セミナーII
  • 化学特別セミナーIII
  • 化学特別セミナーIV
  • 化学特別セミナーII
  • 化学特別セミナーI
  • 化学特別セミナーIV
  • 化学特別セミナーIII
オフィスアワーオフィスアワーは特に設定しませんが、直接質問したい場合随時受付ますので、事前に以下のメールアドレスへメールし、アポイントメントを取ってください。メールアドレス:khirota@tmu.ac.jp
研究室8号館466号室
内線番号内線3535
メールアドレス変換画像
(メールを送信される場合は●を@に変換してください)
研究室サイト等
取組状況平成31年度
researchmap
取組成果平成31年度~平成31年度
ページトップへ