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毛 思鋒

氏 名毛 思鋒マオ スーフォンまお すーふぉん 
職 位助教
所 属東京都立大学都市環境学部 環境応用化学科
都市環境科学研究科 環境応用化学域
 
専門・研究分野分析化学・マイクロ流体・細胞分析
最終学歴・学位工学博士
研究テーママイクロ流体は分子移動において、傑出した構造である。マイクロ化学ペンやマイクロ流体の単細胞分析、マイクロ・ナノセンサーといったマイクロ流体制御の研究を行っている。
研究キーワード分析化学;マイクロ流体;マイクロ化学ペン;単細胞分析
研究業績・著書・
論文、その他
それに準じる業績
研究業績
1. Cell analysis on microchip-mass spectrometry (Cellent MS)
By designing microchips with various functions, cells were cultured on the chip and further analyses were carried to understand cell metabolism, cell apoptosis, cell communications and so on. Relative works (24 papers) were published on Anal. Chem., Lab Chip, Biosens. Bioelectron., Trends in Anal. Chem., and so on. A new method was developed for cell analysis. Moreover, the new instrument based on this technique has come out by cooperating with Shimadzu Company. The designed microfluidic device in one of my publications (Mao et al, Anal. Chem., 2013, 85, 868-876) has become the first generation chip in the Cellent MS.The CELLENT CM-MS has launched in China on May 30th, 2019.
2. Chemical Pen for micro- and nano- fabrication and surface modifications
I started to work on the topic of Chemical Pen with my supervisor (Prof. Katsumi Uchiyama). Three nozzle chemical pen and four nozzle chemical pen were developed for micro- and nano- fabrication on sample surface. The technique can control laminar flow in open space, thus a non-touch, region-selective approach for surface modifications. Using this technique, we succeeded on fabrication nanowires. The resolution has reached 100 nm, which was much higher than all the other methods in related areas. Relative works (14 papers) were published on Chem. Commun (Cover page), Anal. Chem., J. Mater. Chem. C and so on. An new stagey for control of open microfluidics was developed. Moreover, the chemical pen functioned as an open microreactor that was powerful and potential for nanofabrications, and chemical modifications.
3. Open microfluidics for single cell analysis
By designing a two nozzle device, microjet was successfully controlled in open space. This open microfluidics was applied for understanding cell heterogeneity, cell-matrix adhesion and cell-cell adhesion at single-cell resolution. The invasiveness of circling tumor cells (CTCs) were also uncovered by using this approach. As a recently developed method, we have published 12 papers in Angew. Chem., Chem. Sci., Anal. Chem. and so on. A series methods were reported for single cell analysis and subcellular analysis.

研究費(8件)
No. Source of funding Period Funds Personal role
1 日本学術振興会 2020-2021 2860(千円) 研究代表者
2 日本学術振興会 2019-2021 基盤研究(C) 分担者
3 National Natural Science Foundation of China 2020.01-2023.12 677,600 (RMB) Co-Principal Investigator (40%)
4 China Postdoctoral Science Foundation 2018.02 -2019.11 150,000 (RMB) Principal Investigator
5 Beijing Natural Science Foundation 2018.01- 2019.12 80,000 (RMB) Principal Investigator
6 China Postdoctoral Science Foundation 2017.11- 2018.11 50,000 (RMB) Principal Investigator
7 National Natural Science Foundation of China 2018.01- 2022.12 6,292,100 (RMB) Main undertaker
8 National Natural Science Foundation of China 2013.01-2016.12 800,000 (RMB) Main undertaker

特許(12件)
No.     Title                    Country   Type      Application Date Current state
1 一种活体单细胞原位裂解及在线离子化检测质谱接口装置 CHINA National invention patent 2017.11.06 Grant
2 基于微流控芯片的单细胞采样与原位检测质谱接口装置   CHINA National invention patent 2017.11.06 Publication
3 微流控芯片液滴生成装置              CHINA National invention patent 2018.03.05 Publication
4 一种活体单细胞原位切割方法及装置         CHINA National invention patent 2018.03.18 Substantive examination
5 一种细胞外泌物质采样与原位检测方法及装置     CHINA National invention patent 2018.03.18 Substantive examination
6 一种微流控活体组织培养芯片              CHINA National invention patent 2018.12.17 Substantive examination
7 一种活体单细胞原位萃取及在线质谱检测装置及应用   CHINA National invention patent 2018.11.27 Substantive examination
8 微流控芯片液滴生成装置              CHINA National utility model patent 2018.03.15 Grant
9 一种细胞外泌物质采集与原位检测装置         CHINA National utility model patent 2018.04.28 Grant
10 一种活体单细胞原位切割装置              CHINA National utility model patent 2018.05.13 Grant
11 一种活体单细胞原位萃取及在线质谱检测装置     CHINA National utility model patent 2018.11.27 Substantive examination
12 一种微流控活体组织培养芯片             CHINA National utility model patent 2018.12.26 Substantive examination

著書(1件)
Sifeng Mao, Jin-Ming Lin, Chapter 9 Microfluidic chip based Liver Single-Cell Probes, Microfluidics for Single Cell Analysis, Springer.

論文(50件)
[1] Mao, S.; Zhang, W.; Huang, Q.; Khan, M.; Li, H.; Uchiyama, K.; Lin, J.-M*. Angew. Chem. Int. Ed. 2018, 57, 236-240.
[2] Mao, S.; Zhang, Q.; Liu, W.; Huang, Q.; Khan, M.; Zhang, W.; Lin, C.; Uchiyama, K.; Lin, J.-M*. Chem. Sci. 2019, 10, 2081-2087.
[3] Mao, S. †; Zhang, Q. †; Li, H.; Zhang, W.; Huang, Q.; Khan, M.; Lin, J.-M*. Chem. Sci. 2018, 9, 7694-7699.
[4] Mao, S.; Zhang, Q.; Li, H.; Huang, Q.; Khan, M.; Uchiyama, K.; Lin, J.-M*. Anal. Chem. 2018, 90, 9637-9643.
[5] Lin, C.†; Lin, L. †; Mao, S. †; Yang, L.; Yi, L.; Lin, X.; Wang, J.; Lin, Z. X.; Lin, J. M*. Anal. Chem. 2018, 90, 10326-10333.
[6] Mao, S.; Zhang, J.; Li, H.; Lin, J.-M*. Anal. Chem. 2013, 85, 868-876.
[7] Mao, S.; Gao, D.; Liu, W.; Wei, H.; Lin, J.-M*.Lab Chip 2012, 12, 219-226.
[8] Zhang, Y.; Mao, S.*; Suzuki, Y.; Tanaka, Y.; Kawaguchi, M.; Zhang, W.; Zeng, H.; Nakajima, H.; Yang, M.; Uchiyama, K*. Chem. Commun. 2018, 54, 719-722.
[9] Feng, S.†; Mao, S.†; Zhang, Q.; Li, W.; Lin, J.-M*. ACS Sens. 2019, 4, 521-527.
[10] Li, W.†; Mao, S.†; Khan, M; Zhang, Q.; Huang, Q.; Feng, S.; Lin, J.-M*. . ACS Sens. 2019, Doi: 10.1021/acssensors.9b00678.
[11] Mao, S.; Li, W.; Zhang, Q.; Zhang, W.; Huang, Q.; Lin, J.-M*. TrAC, Trends Anal. Chem. 2018, 107, 43-59.
[12] Mao, S.; Zhang, Y.; Li, H.; Zeng, H.; Lin, J.-M.*; Uchiyama, K*. J. Mater. Chem. C 2017, 5, 11666-11671.
[13] Mao, S.; Zhang, Y.; Zhang, Q.; Lin, J.-M.*; Uchiyama, K*. Talanta 2018, 187, 246-251.
[14] Mao, S.; Sato, C.; Suzuki, Y.; Yang, J.; Zeng, H.; Nakajima, H.; Yang, M.; Lin, J.-M.; Uchiyama, K*. Chemphyschem 2016, 17, 3155-3159.
[15] Mao, S.; Zhang, Y.; Zhang, W.; Zeng, H.; Nakajima, H.; Lin, J.-M.*; Uchiyama, K*. Chemphyschem 2017, 18, 2357-2363.
[16] Wang, N.†; Mao, S.†; Liu, W.; Wu, J.; Li, H.; Lin, J.-M.* RSC Adv. 2014, 4, 11919-11926.
[17] Lin, H.; Mao, S.; Zeng, H.; Zhang, Y.; Kawaguchi, M.; Tanaka, Y.; Lin, J.-M.*; Uchiyama, K.*Anal. Chem. 2019, 91, 7346-7352.
[18] Zhang, W.; Mao, S.; He, Z.; Wu, Z.; Zhang, W.; Lin, J.-M.* Anal. Chem. 2019, 91, 5973-5979.
[19] Zhang, Q.; Mao, S.; Khan, M.; Feng, S.; Zhang, W.; Li, W.; Lin, J.-M.* Anal. Chem. 2019, 91, 1644-1650.
[20] He, Z.; Zhang, W.; Mao, S.; Li, N.; Li, H.; Lin, J.-M*. Anal. Chem. 2018, 90, 5540-5545.
[21] Chen, F.; Mao, S.; Zeng, H.; Xue, S.; Yang, J.; Nakajima, H.; Lin, J.-M.; Uchiyama, K*. Anal. Chem. 2013, 85, 7413-7418.
[22] Wei, H.; Li, H.; Mao, S.; Lin, J.-M.* Anal. Chem. 2011, 83, 9306-9313.
[23] Li, W.; Khan, M.; Li, H.; Lin, L.; Mao, S.; Lin, J.-M*. Chem. Commun. 2019, 55, 2166-2169.
[24] Huang, Q.; Mao, S.; Khan, M.; Zhou, L.; Lin, J.-M*. Chem. Commun. 2018, 54, 2595-2598.
[25] Yang, J.; Hida, M.; Mao, S.; Zeng, H.; Nakajima, H.; Uchiyama, K*.2014, 50, 10265-10268.
[26] Wu, Z.; Lin, L.; Khan, M.; Zhang, W.; Mao, S.; Zheng, Y.; Li, Z.; Lin, J.-M*. Chem. Commun. 2018, 54, 11546-11549.
[27] Zhang, W.; He, Z.; Yi, L.; Mao, S.; Li, H.; Lin, J.-M*. Biosens. Bioelectron. 2018, 102, 652-660.
[28] Liu, W.; Wei, H.; Lin, Z.; Mao, S.; Lin, J.-M*. Biosens. Bioelectron. 2011, 28, 438-442.
[29] Zhang, Y.; Zeng, H.*; Mao, S.; Kondo, S.; Nakajima, H.; Kato, S.; Ren, C. L.; Uchiyama, K*. ACS Appl. Mater. Interfaces 2018, 10, 23247-23253.
[30] Khan, M.; Mao, S.; Li, W.; Lin, J.-M*. Chem. Eur. J. 2018, 24, 15398-15420.
[31] Zhang, W.; Mao, S.; Yang, J.; Zeng, H.; Nakajima, H.; Kato, S.; Uchiyama, K*. J. Chromatogr. A 2016, 1477, 127-131.
[32] Wang, J.; Mao, S.; Li, H.-F.; Lin, J.-M*. Anal. Chim. Acta 2018, 1027, 76-82.
[33] Zeng, H.; Zhang, Y.; Mao, S.; Nakajima, H.; Uchiyama, K*. Chem. C 2017, 5, 5877-5881.
[34] Yang, J.; Katagiri, D.; Mao, S.; Zeng, H.; Nakajima, H.; Kato, S.; Uchiyama, K*. J. Mater. Chem. B 2016, 4, 4156-4163.
[35] Khan, M.; Wu, Z.; Mao, S.; Shah, S. N. A.; Lin, J.-M*. J. Mater. Chem. B 2018, 6, 3782-3791.
[36] Li, M.; Mao, S.; Wang, S.; Li, H.*; Lin, J.-M*. Sci. China: Chem. 2018, 61, 1-9.
[37] Zhou, L.; Mao, S.; Huang, Q.; He, X.; Lin, J. M*. Sci. China: Chem. 2018, 61, 1034-1042.
[38] Liu, J.; Gao, D.; Mao, S.; Lin, J.-M*. Sci. China: Chem. 2012, 55, 494-501.
[39] Wang, S.; Mao, S.; Li, M.; Li, H.-F.; Lin, J.-M*. Talanta 2019, 191, 67-73.
[40] Zhong, Z.; Mao, S.; Lin, H.; Lin, J*; Lin, J-M*. Talanta 2019, 204, 6-12.
[41] Zhong, Z.; Mao, S.; Lin, H.; Lin, J.-M.*; Lin, J*. Talanta 2018, 178, 362-368.
[42] Wu, Z.; Khan, M.; Mao, S.; Lin, L.; Lin, J.-M*. Talanta 2018, 181, 217-223.
[43] Shan, S.; He, Z.; Mao, S.; Jie, M.; Yi, L.; Lin, J.-M*. Talanta 2017, 171, 197-203.
[44] Liu, W.; Mao, S.; Wu, J.; Lin, J.-M*. Analyst 2013, 138, 2163-2170.
[45] Huang, Q.; Mao, S.; Khan, M.; Lin, J.-M*. Analyst 2018, 144, 808-823.
[46] Jie, M.; Mao, S.; Liu, H.; He, Z.; Li, H.-F.; Lin, J.-M*. Analyst 2017, 142, 3629-3638.
[47] Chen, J.; Mao, S.; Li, H.; Zheng, M.; Yi, L.; Lin, J.-M.*; Lin, Z*.PLoS One 2017, 12, e0182183.
[48] Jie, M.; Mao, S.; Li, H.; Lin, J.-M*. Chin. Chem. Lett. 2017, 28, 1625-1630.
[49] Yang, J.; Katagiri, D.; Mao, S.; Zeng, H.; Nakajima, H.; Uchiyama, K*. RSC Adv. 2015, 5, 7297-7303.
[50] Li, W.; Khan, M.; Mao, S.; Feng, S.; Lin, J.-M*. J. Pharm. Anal. 2018, Doi: 10.1016/j.jpha.2018.07.005.

学会発表(15件)
[1] Sifeng Mao, Qiang Zhang, Jin-Ming Lin, Adhesion analysis of living single cell in adherent culture using open microfluidics, The 11th International Symposium on Microchemistry and Microsystems, Oral Presentation, Yang Ling, China, May 2019.
[2] Sifeng Mao, Qiang Zhang, Jin-Ming Lin, In situ adhesion analysis of living single cell using open microfluidics, 2018 China-Japan-Korea Symposium on Analytical Chemistry (CJK 2018), Invited Speaker, Zhang Zhou, China, November 2018.
[3] Sifeng Mao, Jin-Ming Lin, Single-cell adhesion analysis of CTCs for revealing tumor metastasis using open microfluidics, Protein and Peptide Therapeutics and Diagnostics: Research and Quality Assurance International Workshop (PPTD), Poster (Best Poster Award), Cheng Du, China, October 2018.
[4] Sifeng Mao, Jin-Ming Lin, Integrated microchip with surface tension valve for cell drug metabolism and cell signaling, 2018 annual meeting of China Pharmaceutical Association pharmaceutical analysis Specialized Committee, Oral Presentation (First prize of outstanding paper), Xi’An, China, August 2018.
[5] Sifeng Mao, Jin-Ming Lin, Vascular Endothelial Cells Enhanced Temozolomide Resistance on Glioma Stem cells Using Chip-MS Platform, The 31st Annual Academic Conference of the Chinese society of Chemistry, Oral Presentation, Hang Zhou, China, May 2018.
[6] Sifeng Mao, Jin-Ming Lin, Development of chemical pen and its applications on chemical modifications and single cell analysis, The 3rd National Conference on Mass Spectrometry, Oral Presentation (Outstanding youth report Award), Xiamen, China, December 2017.
[7] Sifeng Mao, Zhihui Zhong, Jin-Ming Lin, Comparative proteomics of cancer stem cells in osteosarcoma using ultra-high-performance liquid chromatography and Orbitrap Fusion mass spectrometer, The 3rd National Conference on Mass Spectrometry, Poster, Xiamen, China, December 2017.
[8] Sifeng Mao, Siwen Shan, Jin-Ming Lin, Katsumi Uchiyama, Quantitative determination of VEGF165 in cell culture medium by aptamer sandwich based chemiluminescence assay, Beijing Conference and Exhibition on Instrumental Analysis, Oral Presentation, Beijing, China, October 2017.
[9] Sifeng Mao, Jin-Ming Lin, Katsumi Uchiyama, Microchemical pen: an open microreactor for region-selective surface modification, Beijing Conference and Exhibition on Instrumental Analysis, Poster (Best Poster Award), Beijing, China, October 2017.
[10] Sifeng Mao, Chiho Sato, Hulie Zeng, Katsumi Uchiyama*. An microchemical pen for region-selective surface modification, Poster, IFOC8, University of Tokyo, Tokyo, Japan, 8 - 12 March, 2016.
[11] Sifeng Mao, Katsumi Uchiyama, Chemical Pen, Pittcon Conference 2015, Poster, New Oreland, USA, March 2015.
[12] Sifeng Mao, Katsumi Uchiyama, Microfluidic based chemical pen, Japan Analytical and Scientific Instruments Show 2013, Oral Presentation, Makuhari, Japan, September 2014.
[13] Sifeng Mao, Jin-Ming Lin, Imitation of drug metabolism in human liver and cytotoxicity assay using a microfluidic device coupled to mass spectrometric detection, 2012 National Symposium on Micro/NanoScale Bioseparations and Bioanalysis, 7th National Conference on Micro Total Analysis Systems & 3rd International (West Lake) Forum on Microfluidic Analysis, Poster (Best Poster Award), Hangzhou, China, April 2012.
[14] Sifeng Mao, Niejun Wang, Jin-Ming Lin, Cell metabolism and Cytotoxicity Assay on a microchip combining with mass spectrometry, The 14th Beijing Conference and Exhibition on Instrumental Analysis (BCEIA2011), Oral Presentation, Beijing, China, October 2011.
[15] Sifeng Mao, Dan Gao, Jin-Ming Lin, Investigation of drug metabolism and cell cytotoxicity by using an integrated microfluidic device coupled with a mass spectrometer, Poster, The Third National Symposium on Life Analytical Chemistry, Beijing, August 19-22, 2010.
受 賞2018. Best Poster Award, Protein and Peptide Therapeutics and Diagnostics: Research and Quality Assurance International Workshop (PPTD)
2018. First prize for outstanding paper, 2018 Annual Meeting of China Pharmaceutical Association Pharmaceutical Analysis Specialized Committee
2017. Outstanding Postdoctor in the midterm assessment, Tsinghua University
2017. Outstanding Youth Report Award, The 3rd National Conference on Mass Spectrometry
2017. Best Poster Award, Beijing Conference and Exhibition on Instrumental Analysis (BCEIA)
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