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藤井 宣晴

氏 名藤井 宣晴フジイ ノブハルふじい のぶはる
職 位教授
所 属首都大学東京人間健康科学研究科 ヘルスプロモーションサイエンス学域
大学教育センター
 
専門・研究分野分子生物学
最終学歴・学位筑波大学博士課程体育科学研究科 博士(体育科学)
研究テーマ運動が糖尿病の予防・改善に貢献するメカニズムの探索
筋収縮による細胞内情報伝達の変容解析
研究キーワード骨格筋 筋収縮 糖尿病 AMPキナーゼ インスリン 運動 分子生物学 遺伝子工学
研究業績・著書・
論文、その他
それに準じる業績
Yu H, Fujii NL, Toyoda T, An D, Farese RV, Leitges M, Hirshman MF, Mul JD, Goodyear LJ. Contraction stimulates muscle glucose uptake independent of atypical PKC. Physiol Rep 3(11) e12565 2015.

Manabe Y, Ogino S, Ito M, Furuichi Y, Takagi M, Yamada M, Goto-Inoue N, Ono Y, Fujii NL. Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes. Anal Biochem S0003-2697(15)00499-6. 2015.

Goto-Inoue N, Tamura K, Motai F, Ito M, Miyata K, Manabe Y, Fujii NL. A fragmented form of annexin A1 is secreted from C2C12 myotubes by electric pulse-induced contraction. Mol Cell Biochem in press 2015.

Shoji E, Sakurai H, Nishino T, Nakahata T, Heike T, Awaya T, Fujii N, Manabe Y, Matsuo M, Sehara-Fujisawa A. Early pathogenesis of Duchenne muscular dystrophy modelled in patient-derived human induced pluripotent stem cells. Sci Rep 5:12831. 2015

Manabe Y, Takagi M, Nakamura-Yamada M, Goto-Inoue N, Taoka M, Isobe T, Fujii NL. Redox proteins are constitutively secreted by skeletal muscle. J Physiol Sci 64(6):401-9. 2015

Inada A, Inada O, Fujii NL, Fujishima K, Inai T, Fujii H, Sueishi K, Kurachi K: β-cell induction in vivo in severely diabetic male mice by changing the circulating levels and pattern of the ratios of estradiol to androgens. Endocrinology. 155(10): 3829-42. 2014

Miyatake S, Manabe Y, Inagaki A, Furuichi Y, Takagi M, Taoka M, Isobe T, Hirota K, Fujii NL: Macrophage migration inhibitory factor diminishes muscle glucose transport induced by insulin and AICAR in a muscle type-dependent manner. Biochem Biophys Res Commun 444: 496-501, 2014.

Furuichi Y, Goto-Inoue N, Manabe Y, Setou M, Masuda K, Fujii NL: Imaging mass spectrometry reveals fiber-specific distribution of acetylcarnitine and contraction-induced carnitine dynamics in rat skeletal muscles. Biochim Biophys Acta 1837: 1699-1706, 2014.

Furuichi Y, Goto-Inoue N, Fujii NL: Role of carnitine acetylation in skeletal muscle. J Phys Fitness Sports Med 3: 163-168, 2014.

Manabe Y, Takagi M, Nakamura-Yamada M, Goto-Inoue N, Taoka M, Isobe T, Fujii NL: Redox proteins are constitutively secreted by skeletal muscle. J Physiol Sci 64: 401-409, 2014.

Inada A, Inada O, Fujii NL, Fujishima K, Inai T, Fujii H, Sueishi K, Kurachi K: β-cell induction in vivo in severely diabetic male mice by changing the circulating levels and pattern of the ratios of estradiol to androgens. Endocrinology 155: 3829-3842, 2014.

Furuichi Y, Goto-Inoue N, Fujii NL: Role of carnitine acetylation in skeletal muscle. J Phys Fitness Sports Med 3: 163-168, 2014.

Goto-Inoue N, Yamada K, Inagaki A, Furuichi Y, Ogino S, Manabe Y, Setou M, Fujii NL. Lipidomics analysis revealed the phospholipid compositional changes in muscle by chronic exercise and high-fat diet. Sci.Rep., 3, 3267, 2013

Manabe Y, Gollisch KSC, Holton L, Kim Y, Brandauer J, Fujii NL, Hirshman MF, Goodyear LJ. Exercise training-induced adaptations associated with increases in skeletal muscle glycogen content. FEBS J., 280(3):916-926, 2013

Tsuchiya M*, Manabe Y* (*contributed equally), Yamada K, Furuichi Y, Hosaka M, Fujii NL. Chronic exercise enhances insulin secretion ability of pancreatic islets without change in insulin content in non-diabetic rats. Biochem.Biophys.Res.Commun., 430(2):676-682, 2013

Manabe Y, Miyatake S, Takagi M, Nakamura M, Okeda A, Nakano T, Hirshman MF, Goodyear LJ, Fujii NL. Characterization of an Acute Muscle Contraction Model using Cultured C2C12 Myotubes. PLoS ONE,7(12):e52592, 2012

Goto-Inoue N, Setou M, Fujii NL. Visualization of metabolite change in skeletal muscle by contraction using imaging mass spectrometry. J.Phys.Fit.Sport.Med., 1(2): 347-350. 2012

Goto-Inoue N, Manabe Y, Miyatake S, Ogino S, Morishita A, Hayasaka T, Masaki N, Setou M, Fujii NL. Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry. Anal.Bioanal.Chem., 403(7):1863-1867, 2012

Henstridge DC, Bruce CR, Pang CP, Lancaster GI, Allen TL, Estevez E, Gardner T, Weir JM, Meikle PJ, Lam KS, Xu A, Fujii N, Goodyear LJ, Febbraio MA. Skeletal muscle-specific overproduction of constitutively activated c-Jun N-terminal kinase (JNK) induces insulin resistance in mice. Diabetologia. 55(10):2769-2778. 2012

Toyoda T, An D, Witczak CA, Koh HJ, Hirshman MF, Fujii N, Goodyear LJ. Myo1c regulates glucose uptake in mouse skeletal muscle.  J.Biol.Chem. 2011, 286(6):4133-40.

Sugita S, Kamei Y, Akaike F, Suganami T, Kanai S, Hattori M, Manabe Y, Fujii N, Takai-Igarashi T, Tadaishi M, Oka J, Aburatani H, Yamada T, Katagiri H, Kakehi S, Tamura Y, Kubo H, Nishida K, Miura S, Ezaki O, Ogawa Y. Increased systemic glucose tolerance with increased muscle glucose uptake in transgenic mice overexpressing RXRγ in skeletal muscle. PLoS One. 2011;6(5):e20467.

Koh HJ, Toyoda T, Fujii N, Jung MM, Rathod A, Middelbeek RJ, Lessard SJ, Treebak JT, Tsuchihara K, Esumi H, Richter EA, Wojtaszewski JF, Hirshman MF, and Goodyear LJ, Sucrose nonfermenting AMPK-related kinase (SNARK) mediates contraction-stimulated glucose transport in mouse skeletal muscle. Proc Natl.Acad.Sci. USA. 2010; 107(35): 15541-6.

Jessen N, Koh HJ, Folmes CD, Wagg C, Fujii N, Lofgren B, Wolf CM, Berul CI, Hirshman MF, Lopaschuk GD, and Goodyear LJ, Ablation of LKB1 in the heart leads to energy deprivation and impaired cardiac function. Biochim Biophys Acta. 2010; 1802(7-8): 593-600.

An D, Toyoda T, Taylor EB, Yu H, Fujii N, Hirshman MF, and Goodyear LJ, TBC1D1 regulates insulin- and contraction-induced glucose transport in mouse skeletal muscle. Diabetes. 2010; 59(6): 1358-65.

Witczak CA, Jessen N, Warro DM, Toyoda T, Fujii N, Anderson ME, Hirshman MF, and Goodyear LJ, CaMKII regulates contraction- but not insulin-induced glucose uptake in mouse skeletal muscle. Am.J.Physiol. Endocrinol.Metab. 2010; 298(6): E1150-60.

Fujii N. Molecular mechanism of glucose transport in skeletal muscle. Adv.Exer.Sport.Physiol. 2008; 14(3): 45-47

Fujii N, Ho RC, Manabe Y, Jessen N, Toyoda T, Holland WL, Summers SA, Hirshman MF, Goodyear LJ. Ablation of AMP-activated protein kinase alpha2 activity exacerbates insulin resistance induced by high-fat feeding of mice. Diabetes. 2008 Nov;57(11):2958-66

Higaki Y, Mikami T, Fujii N, Hirshman MF, Koyama K, Seino T, Tanaka K, Goodyear LJ. Oxidative stress stimulates skeletal muscle glucose uptake through a phosphatidylinositol-3-kinase-dependent pathway. Am.J.Physiol. (Endocrinol Metab.) 2008; May;294(5):E889-97.

Taylor EB, An D, Kramer HF, Yu H, Fujii N, Roeckl KS, Bowles N, Hirshman MF, Xie J, Feener EP, Goodyear LJ. Discovery of TBC1D1 as an insulin-, AICAR-, and contraction-stimulated signaling nexus in mouse skeletal muscle. J.Biol.Chem. 2008; 283(15):9787-96.

Kramer HF, Taylor EB, Witczak CA, Fujii N, Hirshman MF, Goodyear LJ, Calmodulin-binding domain of AS160 regulates contraction- but not insulin-stimulated glucose uptake in skeletal muscle. Diabetes 2007; 56(12):2854-62.

Ho RC, *Fujii N, Witters LA, Hirshman MF, Goodyear LJ. Dissociation of AMP-activated protein kinase and p38 mitogen-activated protein kinase signaling in skeletal muscle.

Rockl KS, Hirshman MF, Brandauer J, Fujii N, Witters LA, Goodyear LJ. Skeletal muscle adaptation to exercise training: AMP-activated protein kinase mediates muscle fiber type shift. Diabetes 2007; 56(8):2062-9.

Fujii N, Seifert MM, Kane EM, Peter LE, Ho RC, Winstead S, Hirshman MF, Goodyear LJ. Role of AMP-activated protein kinase in exercise capacity, whole body glucose homeostasis, and glucose transport in skeletal suscle. - Insight from analysis of a transgenic mouse model - Diabetes Res.Clin.Pract. 2007; 77:S92-8.

Witczak CA, Fujii N, Hirshman MF, Goodyear LJ. Ca2+/calmodulin-dependent protein kinase kinase-alpha regulates skeletal muscle glucose uptake independent of AMP-activated protein kinase and Akt activation. Diabetes 2007; 56(5):1403-9.

Fujii N, Jessen N, Goodyear LJ. AMP-activated Protein Kinase and the Regulation of Glucose Transport. Am.J.Physiol. (Endcrinol.Metab.) 2006; 291(5):E867-77

Witczak CA, Hirshman MF, Jessen N, Fujii N, Seifert MM, Brandauer J, Hotamisligil GS, Goodyear LJ. JNK1 deficiency does not enhance muscle glucose metabolism in lean mice. Biochem.Biophys.Res.Commun. 2006; 350(4):1063-8

Koh H, Arnolds DE, Fujii N, Hirshman MF, Goodyear LJ. Skeletal muscle-selective knockout of LKB1 increases insulin sensitivity, improves glucose homeostasis, and decreases TRB3. Mol.Cell Biol. 2006; 26(22):8217-27

Kramer HF, Witczak CA, Taylor EB, Fujii N, Hirshman MF, Goodyear LJ. AS160 regulates insulin- and contraction-stimulated glucose uptake in mouse skeletal muscle. J.Biol.Chem. 2006; 281(42):31478-85

Kramer HF, Witczak CA, Fujii N, Jessen N, Taylor EB, Arnolds DE, Sakamoto K, Hirshman MF, Goodyear LJ. Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle. Diabetes 2006; 55(7):2067-76

Sakamoto K, Arnolds DE, Fujii N, Kramer HF, Hirshman MF, Goodyear LJ. Role of Akt2 in contraction-stimulated cell signaling and glucose uptake in skeletal muscle. Am.J.Physiol. (Endocrinol.Metab.) 2006; 291(5):E1031-7

Yu H, Hirshman MF, Fujii N, Pomerleau JM, Peter LE, Goodyear LJ. Muscle-specific overexpression of wild type and R225Q mutant AMP-activated protein kinase gamma3 Subunit differentially regulates glycogen accumulation. Am.J.Physiol. (Endocrinol.Metab.) 2006; 291(3):E557-65.

Aschenbach WG, Ho RC, Sakamoto K, Fujii N, Li Y, Kim YB, Hirshman MF, Goodyear LJ. Regulation of dishevelled and beta-catenin in rat skeletal muscle: an alternative exercise-induced GSK-3beta signaling pathway. Am.J.Physiol. (Endocrinol.Metab.) 2006; 291 (1): E152-8.

Fujii N, Hirshman MF, Kane EM, Ho RC, Peter LE, Seifert MM, Goodyear LJ. AMP-activated protein kinase alpha2 activity is not essential for contraction-and hyperosmolarity-induced glucose transport in skeletal muscle. J.Biol.Chem. 2005; 280 (47): 29033-29041.

Pold R, Jensel LS, Jessen N, Buhl ES, Schmitz O, Flyvbjerg A, Fujii N, Goodyear LJ, Gotfredsen CF, Brand CL, Lund S. Long-term AICAR administration and exercise prevents diabetes in ZDF rats. Diabetes 2005; 54(4):928-34

Musi N, Hirshman MF, Arad M, Xing Y, Fujii N, Pomerleau J, Ahmad F, Berul CI, Seidman JG, Tian R, Goodyear LJ. Functional Role of AMP-activated protein kinase in the heart during exercise. FEBS Lett. 2005; 579(10):2045-55

Fujii N, Aschenbach WG, Musi N, Hirshman MF, Goodyear LJ. Regulation of glucose transport by the AMP-activated protein kinase. Proc.Nutr.Soc. 2004; 63: 205-210

Alcazar O, Ho R, Fujii N, Goodyear LJ. cDNA cloning and functional characterization of a novel splice variant of c-Cbl associated protein (CAP) from mouse skeletal muscle. Biochem.Biophys.Res.Comm. 2004; 317(1): 285-93

Fujii N, Boppart MD, Dufresne SD, Crowley PF, Jozsi AC, Sakamoto K, Kim S, Miyazaki H, Hirshman MF, Goodyear LJ. Overexpression or ablatiation of JNK in skeletal muscle has no effect on glycogen synthase activity. Am.J.Physiol. (Cell Physiol.) 2004; 287(1): C200-8

Ho R, Alcazar O, Fujii N, Hirshman MF, Goodyear LJ. p38gamma MAPK regulation of glucose transporter expression and glucose uptake in L6 myotubes and mouse skeletal muscle. Am.J.Physiol. (Regul.Integr.Comp.Physiol.) 2004; 286(2): R342-9

Yu H, Fujii N, Hirshman MF, Pomerleau JM, Goodyear LJ. Cloning and characterization of mouse 5’AMP-activated protein kinase gamma3 subunit. Am.J.Physiol. (Cell Physiol.) 2004; 286(2): C283-92

Iemitsu M, Miyauchi T, Maeda S, Sakai S, Fujii N, Miyazaki H, Kakinuma Y, Matsuda M, Yamaguchi, I. Cardiac hypertrophy by hypertension and exercise training exhibits different gene expression of enzymes in energy metabolism. Hypertens.Res.2003; 26(10): 829-37

Xing Y, Musi N, Fujii N, Zou L, Luptak I, Hirshman MF, Goodyear LJ, Tian R. Glucose metabolism and energy homeostasis in mouse hearts overexpressing dominant negative alpha 2 subunit of AMP-activated protein kinase. J.Biol.Chem.2003; 278(31): 28372-28377

Kaneto H, Xu G, Fujii N, Kim S, Bonner-Weir S, Weir GC. Involvement of c-Jun N-terminal kinase in oxidative stress-mediated suppression of insulin gene expression. J.Biol.Chem. 2002; 277(33): 30010-30018

Aschenbach WG, Hirshman MF, Fujii N, Sakamoto K, Howlett KF, Goodyear LJ. Effect of AICAR treatment on glycogen metabolism in skeletal muscle. Diabetes 2002; 51(3): 567-573

Iemitsu M, Miyauchi T, Maeda S, Sakai S, Kobayashi T, Fujii N, Miyazaki H, Matsuda M, Yamaguchi I. Physiological and pathological cardiac hypertrophy induce different molecular phenotypes in the rat. Am.J.Physiol. (Regul Integr Comp Physiol ) 2001; 281(6): R2029-2036

Zhou G, Myers R, Li Y, Chen Y, Shen X, Fenyk-Melody J, Wu M, Ventre J, Doebber T, Fujii N, Musi N, Hirshman MF, Goodyear LJ, Moller DE. Role of AMP-activated protein kinase in mechanism of metformin action. J.Clin.Invest. 2001; 108(8): 1167-1174

Musi N, Fujii N, Hirshman MF, Ekberg I, Froberg S, Ljungqvist O, Thorell A, Goodyear LJ. AMP-activated protein kinase (AMPK) is activated in muscle of subjects with type 2 diabetes during exercise. Diabetes 2001; 50(5): 921-927

Musi N, Hayashi T, Fujii N, Hirshman MF, Witter LA, Goodyear LJ. AMP-activated protein kinase activity and glucose uptake in rat skeletal muscle. Am.J.Physiol. (Endocrinol. Metab.) 2001; 280 (5): E677-684

Higaki Y, Hirshman MF, Fujii N, Goodyear LJ. Nitric oxide increases glucose uptake through a mechanism that is distinct from the insulin and contraction pathways in rat skeletal muscle. Diabetes 2001; 50(2): 241-247

Fujii N, Hayashi T, Hirshman MF, Smith JT, Habinowski SA, Kaijser L, Mu J, Ljungqvist O, Birnbaum MJ, Witters LA, Thorell A, Goodyear LJ. Exercise induces isoform-specific increases in 5’ AMP-activated protein kinase in human skeletal muscle. Biochem.Biophys.Res.Comm. 2000; 273(3): 1150-1155

Hayashi T, Hirshman M, Fujii N, Habinowski SA, Witters LA, Goodyear LJ. Metabolic stress and altered glucose transport: activation of AMP-activated protein kinase as a unifying coupling mechanism. Diabetes 2000; 49(4): 527-531

Tanaka M, Tsuchida S, Imai T, Fujii N, Miyasaki H, Ichiki T, Naruse M, Inagami T. Vascular response to angiotensin II is exaggerated through an upregulation of AT1 receptor in AT2 knockout mice. Biochem.Biophys.Res.Commun. 1999; 258(1): 194-198

Kotani E, Sugimoto M, Kamata H, Fujii N, Saitoh S, Usuki S, Kubo T, Song K, Miyazaki M, Murakami K, Miyazaki H. Biological roles of angiotensin II via its type2 receptor during rat follicle atresia. Am.J.Physiol. (Endocrinol. Metab.) 1999; 276(39): E25-33

Fujii N, Nagashima S, Yukawa K, Miyauchi T, Maki S, Sakai S, Murakami K, Miyazaki H. Hypotensive effects of an angiotensin II type 1 receptor antagonist differ between exercised and sedentary rats aged from 4 to 19 weeks. Jpn.J.Physiol. 1998(3); 48: 215-218

Fujii N, Homma S, Yamazaki F, Sone R, Ikegami H, Murakami K, Miyazaki H. b-adrenergic receptor number in human lymphocytes is inversely correlated with aerobic capacity. Am.J.Physiol. (Endcrinol. Metab.) 1998; 274(37): E1106-1112

Fujii N, Shibata T, Homma S, Ikegami H, Murakami K, Miyazaki H. Exercise-induced changes in b-adrenergic receptor mRNA level measured by competitive RT-PCR. J.Appl.Physiol. 1997; 82(6): 1926-1931

Fujii N, Shibata T, Yamazaki F, Sone R, Fukuoka Y, Nabekura Y, Ikegami H, Murakami K, Miyazaki H. Exercise-induced change in b-adrenergic receptor number in lymphocytes from trained and untrained men. Jpn.J.Physiol. 1996; 46(5): 389-395

Fujii N, Tanaka M, Ohnishi J, Yukawa K, Takimoto E, Shimada S, Naruse M, Sugiyama F,Yagami K, Murakami K, Miyazaki H. Alterations of angiotensin II receptor contents in hypertrophied hearts. Biochem.Biophys.Res.Comm. 1995; 212(2): 326-333

Fujii N, Miyazaki H, Homma S, Ikegami H. Dynamic exercise induces translocation of b-adrenergic receptors in human lymphocytes. Acta Physiol.Scand. 1993; 148(4): 463-464
受 賞
主な学会活動
社会等との関わり著書等

「糖尿病治療のニューパラダイム 第1巻」加来浩平, 加来浩平, 松田昌文, 池上博司, 宇都宮一典 編集, 医薬ジャーナル社, in Press 2015, 眞鍋康子, 藤井宣晴 (運動による血糖降下のメカニズム 担当)

「Musculoskeletal Disease Associated With Diabetes Mellitus」Masaaki Inaba Eds, Springer, in press 2015 Fujii NL (Overview. In in Press)

「骨格筋代謝と疾患の最前線 -骨格筋ワールドへの誘い-」 実験医学 32, 1326-1331. 2014, 藤井宣晴

「糖尿病・代謝疾患の分子標的と治療薬辞典」 春日正人監修,綿田裕孝・松本道宏編著, 羊土社, 2013, 眞鍋康子、藤井宣晴(3章 筋肉 担当)

「運動療法ガイド 改訂第5版」  井上一 、武藤 芳照、福田濶 編集  森克人、藤井宣晴(糖尿病 担当) 日本医事新報社 2012年

「運動処方の指針 -運動負荷試験と運動プログラム- 原著第8版」 American College of Sports Medicine. 日本体力医学会体力医学編集委員会 監訳;藤井宣晴(Section IV 付録D pp323-376担当)  南江堂 2011年

「健康と運動の疫学入門」 熊谷秋三(責任編集者); 藤井宣晴 他(編集委員) 医学出版 2008年

「運動生理・生化学辞典」 大野秀樹 他(編者); 藤井宣晴(分担執筆) 大修館書店 2001年

「身体機能の調節性」 池上晴夫 編; 藤井宣晴 第12章 運動と自律神経系 朝倉書店 1997年
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担当科目
  • 健康スポーツ科学
  • 身体運動演習
  • 身体運動学
  • 人間健康科学概論
  • 環境生理生化学
  • 生理学実験(身体運動科学)
  • 適応科学特別講義
  • 運動分子生物学特論
  • 運動生理学特論
  • ヘルスプロモーションサイエンス特講Ⅰ
  • ヘルスプロモーションサイエンス特別講義Ⅰ
  • 健康スポーツ科学
  • 身体運動演習
  • 身体運動学
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